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Practice parameter update: the care of the patient with amyotrophic lateral sclerosis: drug, nutritional, and respiratory therapies (an evidence-based review): report of the Quality Standards Subcommittee of the American Academy of Neurology

Blood was collected from each animal at baseline and at 0.083, 0.25, 0.5, 1, 2, 4, 8, and 24 h after dosing into K 2 EDTA-coated tubes (three animals per time point, with three time points sampled per animal)

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If you need a comprehensive overview of MCAS, I encourage you to read my article: Mast Cell Activation Syndrome and Histamine: When Your Immune System Runs Rampant