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In brief, 20 l of Viability/Cytotoxicity Reagent containing both GF-AFC was added to each well, followed by 30 sec mixing at room temperature and incubation at 37C for 30 min

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These complementary pathways allow investigators to explore how cytoskeletal status, vascular signaling, and inflammatory mediators interact in broader cellular response networks

The safety comes from medical supervision: proper dosing, sterile technique, and professional monitoring