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After adding 1 mM DTT, 10 mM MgCl 2, and 2 mM ATP to remove contamination of heat shock proteins, the eluted target protein was concentrated with a 50 kDa cut-off concentrator (Millipore) and further purified by the Superose 6 increase 10/300 GL column (Cytiva) running in a buffer containing 20 mM HEPES pH 8.0, 150 mM NaCl, 0.025% DDM, and 0.0025% CHS

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During the preparation of this work, RF and CV utilized the Sora tool, available at the time through ChatGPT, to generate illustrative images of neural cells displaying a range of morphologies and cellular extensions, effectively highlighting their phenotypic diversity