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We ranked the models by their predicted local distance different test (pLDDT) and selected the top two models of different linker lengths (20 and 30 amino acids) to test experimentally

Regarding liver and kidney damage in MTX treatment, Bedoui stated that MTX hepatotoxicity may occur due to the depletion of folate reserves and MTX-polyglutamate formation in the liver (Reference Bedoui, Guillot and Slambarom41)