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RatCol collagen, Advanced Biomatrix) and cultured for 7 days in Williams E medium supplemented with CM4000 cell maintenance supplement (ThermoFisher Scientific)

The results, as shown in Figures 5A,B, show that the concentrations of H 2 O 2 in the culture medium of both the single cells and the 3D spheroids increase with time after the AA stimulation

10.1016/j.neuron.2009.01.033 [DOI] [PMC free article] [PubMed] [Google Scholar] Mosharov EV, Staal RGW, Bov J, Prou D, Hananiya A, Markov D, Poulsen N, Larsen KE, Moore CMH, Troyer MD, Edwards RH, Przedborski S, Sulzer D, 2006