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Interestingly, in a process similar to step 1 of the CSD evolution in D2, modification of the canonical Ig disulfide between strands B and F has also occurred in D1 of some fish CD4 proteins (trout and fugu), which contain a distinct unpaired Cys in strand F, but lack the second Cys in strand B that forms a disulfide in mammalian and bird CD4 molecules

Miyahara S, Ohuchi M, Nomura M, Hashimoto E, Soga T, Saito R, et al

It was further proposed that a minimum length of the ligand should be 10 to 11 of - linked glucose residues (163), and Dectin-1 - glucan complex might get more robust in the presence of divalent ions (142)

Handling occlusions in cluttered backgrounds and improving model adaptability in dynamic or variable scenes remain critical areas for future research