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However, when these oxidants accumulate within red cells, hemoglobin and other proteins are oxidized, leading to loss of function and RBC death

The first mainly depends on the genetic inheritance, and the other depends on the exposure intensity and frequency

Briefly, a RT-PCR-nested amplification was performed, and purified PCR products were quantified using the PicoGreen assay (Invitrogen, Carlsbad, CA, USA), and quality analysed using the BioAnalyzer DNA 1000 LabChip (Agilent, Santa Clara, CA, USA) prior to sequencing

Duration: Most researchers run this combination for 12 to 16 weeks before taking a break to assess results and prevent desensitization