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Using a centrifugation process for 30 min at 15,000 rpm (REMI, Mumbai, India) and cold distilled water washing, PEGylated-DOX-loaded-PLGA-NPs were made apart from bulk aqueous phase and freeze dried (Labconco, TriadTM, Kansas City, MO)

The matrix effect calculated for DOX (A/B 100) at different levels showed a %CV (n = 6) of 3.12 for LQC and 3.37 for HQC whereas the %CV 84.89%), linearity, accuracy and precision alongwith stability (bench-top, long term, freeze thaw stability and post processing stability) and matrix effect, were found in acceptable range

Int J Mol Sci (2020) 21(19):7057