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Circulating markers of intestinal epithelial injury, including I-FABP, D-lactate, and citrulline, have been proposed as early indicators of intestinal barrier disruption

10.1038/nrneurol.2010.71 52 SerafiniG.PompiliM.InnamoratiM.DwivediY.BrahmachariG.GirardiP

Slides were mounted with a mounting-solution reagent containing DAPI (Vector Lab, Burlingame, CA, USA) and then analyzed using a Zeiss Axiovert 200 fluorescence microscope with AXIOVISION 4.2 software (Carl Zeiss, Jena, Germany)