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The initial rate of the reaction was determined from the kinetic curves, and the calculation of the specific activity of ACOX1 is expressed in units of H 2 O 2 produced per minute per milligram of protein (1 M dH 2 O 2 corresponds to 115.75 RFU) according to the following formula: Evaluation of the ROS production in cells Intracellular ROS production levels were measured using 2,7-dichlorodihydrofluorescein diacetate (H 2 DCFDA) assay [28]

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Data analysis and visualization were performed using Perseus software (Max Plank Institute, Martinsried) and the statistical environment R

Antioxidants are substances that can prevent damage to cells caused by free radicals, potentially harmful molecules that the body produces as a reaction to environmental and other pressures