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Evidence that glutathione and the glutathione system efficiently recycle 1-cys sulfiredoxin in vivo

10.1007/s10295-010-0730-9 14 BazinetL.FirdaousL

MurU: to 100 mM Tris buffer pH 7.9, 2.0 mM, 1a to 3a MurNAc-1P substrates, 4.0 mM UTP (Sigma-Aldrich) and 0.5 U of bakers yeast inorganic pyrophosphatase (Sigma-Aldrich) was added 1.0 g purified MurU enzyme per 100 l reaction sample