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This condition, known as melena, can stem from the surgery itself or the patients health before surgery

Cells were harvested by centrifugation at 650 x g for 7 min, and washed twice with Wash Buffer (35 mM HEPES/NaOH pH 7.5, 140 mM NaCl, 11 mM glucose) and once with Extraction Buffer (20 mM HEPES/KOH pH 7.5, 45 mM KOAc, 45 mM KCl, 1.8 mM Mg(OAc) 2 , 1 mM DTT)