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Subcutaneous tissue and muscles were separated by blunt dissection with forceps, the dura mater was blotted dry with a sterile cotton swab, and CSF was collected using a sterile pulled capillary to penetrate the cisterna magna (through the dura mater, lateral to the arteria dorsalis spinalis) and immediately snap-frozen in liquid nitrogen and stored at 80 C

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doi: 10.3748/wjg.v20.i44.16452, 21