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As such, testing macrophage or epithelial cell lines can only represent the isolated response of these cells to a microenvironment into which hAAT is introduced, allowing the extraction of insights accordingly, while the in-vivo models entail the responses of all other cell types at the same time, with the addition of systemic aspects such as hAAT replenishment and clearance

Szab A, Vncsa S, Hegyi P, Vradi A, Forintos A, Filipov T, et al

Hydrogels as smart biomaterials As previously discussed, hydrogels have emerged as a transformative platform in biomedical engineering, combining structural versatility, biocompatibility, and functional adaptability to address challenges in precision therapy [125]

Absorbance at 450 nm was measured using a microplate reader, with total NAD + /NADH concentration (A) determined by comparison against an NADH standard curve