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Description

Notably, S309 has been previously identified as a phosphorylated residue in RIPK1 in a large-scale mass spectrometry-based proteogenomic study [47]

The best fractions of protein peak were pooled, concentrated to 10 mg/mL with an Amicon Centrifugal filter (Millipore), flash-frozen in liquid nitrogen, and stored at 80 C for crystallization and activity assay