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Directions: For optimal results, consume 1 serve morning and night in 100ml of water

Thus, when using alcohol models, strict controls are necessary to avoid misinterpretation of the data

However, we have not codified this policy in prior rules

Protein extraction, quantification, and Western blot analysis Cell lysates were prepared in RIPA lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 100 mM NaF, 2 mM EGTA, 1% Triton X-100) supplemented with protease inhibitors cocktail (Merck Sigma #P8340) and phosphatase inhibitor cocktail (Merck Sigma #P5726), and subsequently centrifuged at 14,000 rpm and 4 C for 10 min