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However, when GSH is excessively depleted and cannot neutralize all reactive intermediates, the unconjugated electrophilic species can irreversibly bind to critical cysteine residues of functional hepatic proteins

Despite the effectiveness of immune checkpoint inhibitors (ICIs), some patients do not respond to them and there may be adverse inflammatory reactions similar to autoimmune diseases, promoting research into combination therapies for cancer treatment [144]

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ELISA assay After euthanizing the mice, the plasma samples collected from experimental animals blood were obtained by centrifugation at 3000 rpm for 15 min