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doi: 10.1016/B978-0-12-802728-8.00001-1 275 SemovaI.CartenJ

Alkylation of the proteins was achieved by adding 3.5 l of 100 mM iodoacetamide (IAM) to the samples followed by agitation using a shaker (1200 rpm) at 37 C for 15 min

[20] ROS can form as a result of drought, injury, herbicides and pesticides, ozone, plant metabolic activity, nutrient deficiencies, photoinhibition, temperature above and below ground, toxic metals, and UV or gamma rays