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Note on the tolerance/diminishing-effect claim: the widely repeated effect fades after 4 to 6 weeks figure appears as repeated online claims, not in any primary study I could locate

The collision of replication forks with immobilised topoisomerase 1, which can be trapped on the DNA by camptothecin (CPT), triggers the phosphorylation of the DNA damage checkpoint kinase Chk1 at serine 345 by Rad3 26