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Diffusion dynamics across the FB-OOC To determine the flow of materials and their kinetics, as well as to measure cell barrier function, dextran beads (molecular weight = 3,000 kDa) were utilized to determine hydrostatic pressure across the device

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As previously described 21 , four microliters (4 g) of reconstituted peptides were loaded and washed on a Pepmap100 C18 trap (5 m, 0.3 5.0 mm, Thermo Fisher Scientific, Waltham, MA, USA) at a flow rate of 20 L/min