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After washing sessions in PBS for 3 10 min, these were then incubated in donkey Alexa-488 and 594 conjugated secondary antibodies (1:100 dilutions, Thermofisher, UK) for 1 h at RT

256 Therefore, the administration of KD may be a potential approach to enhance the therapeutic efficacy of chemotherapy

This dual action not only improves tumor regression but also strengthens immune responses by triggering both innate and adaptive immunity (Fig

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