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PMID: Summary Keywords autoimmune diseases, butyrate, clostridium, immune interaction, immunotherapy Citation Zhan J-Y, Wang W-R, Zhan T, Yang J-Y, Liang Y, Yu R-H and Wang X-H (2026) Recent advances in the positive role of Clostridium in autoimmune diseases: as a crucial regulator of restoring immune-metabolic equilibrium

In brief, standard solution (0, 15, 30, 60, 90, 120 and 150 mg/l creatinine in dH 2 0) or serum was added to a working solution (0.1 g picric acid in 50 ml dH 2 0 and 50 ml 0.4 NaOH in 4 C) (1:10), incubated at RT for 15 min and measured at 492 nm

A common laboratory approach is to add 2 mL of bac water to a 10mg vial, yielding a 5 mg/mL working concentration

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