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Briefly, roGFP2 expression was induced as described above for 16 h at 20 C

With regard to azoles, Huppertz et al

The recent review by Mao (1), discussed the bioactive compound and bioactivities of ginger, including antioxidant activity, anti-inflammatory activity, antimicrobial activity, neuroprotection, anti-obesity activity, anti-diabetic activity, protective effect against respiratory disorders, also the other bioactivities of ginger (hepatoprotective and antiallergic effects) (1)

Additionally, after denaturation by boiling in loading buffer, the total protein in cell lysate was separated by 15% SDS-PAGE, transferred from gel to nitrocellulose membrane (Millipore, USA) at 250 mA for 2 h, and blocked with by 5% non-fat milk for 1 h