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Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.The handling editor declared a past co-authorship with the authors

The replacements of Tyr 2 by residues with restricted flexibility were deleterious for activity

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For example, starting at 0.25mg for 4 weeks (using 1mg total) and then switching to 0.5mg for the remaining time would use 1mg plus roughly 1.5mg in the next 3 weeks, totaling 2.5mg from one vial within the 28-day stability window