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After treatment with pure acetone for 20 min, the cells were treated with a mixture of embedding agent and acetone (V/V=1/1 for 1 h, then V/V=3/1 for 3 h) and pure embedding agent overnight

Reported ribosomal occupancy of RNAs indicated on the one side presence of different protein isoforms and regulatory upstream open reading frames ORFs (uORFs) from the mRNAs, and on the other, more exciting side, new ways of translational regulation and possible micropeptide production from lncRNAs (Morris and Geballe, 2000

2004 Jun;26(3):267-71

Centrifugar a 4000 r/min durante 5 min, tomar la fase acuosa superior y filtrarla a travs de un filtro de 0.22 m, para su determinacin por cromatografa lquida