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Note: Avoid mixing with high-concentration Vitamin C (L-ascorbic acid) or strong acids in the same step, as they may destabilize the copper ions

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PubMed Seiwerth S, et al

For histological analysis, the femoral and tibial condyles were fixed in 10% neutral buffered formalin, decalcified with 20% EDTA, embedded in paraffin, and sectioned into 5-m thick frontal slices