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Krometis said excess incubation can cause some of the distinctive metallic sheen of coliform colonies to fade, leading analysts to bypass them when making selection for a battery of confirmatory tests required if the presence of coliform is found, a so-called false negative. She did not feel that the extra hour would be particularly damaging to the overall analysis, but, she said, its not great practice. The EPA, through spokesperson Robledo, expressed a bit more concern, saying the required follow-up confirmation testing performed in case of a preliminary coliform detection during the initial round whose times are reported to the state could be spoiled

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The tumor specimens were cut to about 4 m sections and then embedded in paraffin for the immunohistochemistry assay