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Urine was collected before the end of each of the following intervals: 04 h, 48 h, 812 h, 1224 h and 2436 h after NAC administration on Day 1 and Day 2, and 04 h, 48 h, 812 h and 1224 h on Day 6 and Day 7

Hashemi M, Taheriazam A, Daneii P, Hassanpour A, Kakavand A, Rezaei S, Hejazi ES, Aboutalebi M, Gholamrezaie H, Saebfar H, Salimimoghadam S, Mirzaei S, Entezari M, Samarghandian S

Quantitative real-time PCR Total RNA was extracted from homogenized tissue or cell culture and reverse transcribed as described previously (Schleicher et al, 2016)

The organisms can also withstand being completely dried out, frozen to a temperature just above absolute zero (minus 458 degrees Fahrenheit, when all molecular motion ceases), heated to a temperature higher than 300 degrees Fahrenheit, and exposed to radiation thousands of times higher than humans could tolerate