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MIF-driven activation of macrophages induces killing of intracellular Trypanosoma cruzi dependent on endogenous production of tumor necrosis factor, nitric oxide and reactive oxygen species

Ohmura H, Hanamura F, Okumura Y, Ando Y, Masuda T, Mimori K, et al

If the biotin is conjugated to an active site of an enzyme, or at the interface with an important interaction, then biotinylation may disrupt that proteins function