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Observations of altered intestinal permeability have been reported in both patients diagnosed with RA and individuals with preclinical RA [72, 73]

After removing the DEPC-water and adding 15 mL Triethanolamine-acetic anhydride solution for 10 min at RT, slides were washed in DEPC-PBS at room temperature for 5 min and pre-hybridize in hybridization solution (50% Formamide, 5 SSC, 0.3 mg/mL Yeast tRNA, 100 g/mL Heparin, 1 Denhardts Solution, 0.1% Tween 20, 0.1% CHAPS, 5 mM EDTA) for 1-4 h in a 65 C water bath

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The antidepressant fluoxetine (Prozac) modulates estrogen signaling in the uterus and alters estrous cycles in mice