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Briefly, cells were fixed with the Fixative Solution III for 15 min at room temperature, and incubated with the Staining Solution Mix overnight at 37 C in a termostated room (with air CO 2 concentration, to avoid pH changes in the samples that would inhibit the enzymatic reaction)

In warmer seasons, the surface water is warmer and less dense, while deeper water remains cooler and denser, leading to stable layers

On the contrary, in four studies (9.5%), no significant changes were detected between the antioxidant therapy group and the placebo group

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