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doi: 10.1016/j.ceca.2019.06.004 346

Moreover, ALA exerts antioxidant effects via a dual mechanism: (i) rapid redox-cycling between its disulfide (ALA) and dihydrolipoate (DHLA) forms, which directly quenches ROS without transcriptional activation, and (ii) secondary upregulation of the Nrf2 pathway and suppression of NF-B nuclear translocation (Ghibu et al., 2009)

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The supernatant was transferred to a new Eppendorf tube for derivatization with 3-nitrophenylhydrazine (25 mM), N-(3-Dimethylaminopropyl)-N-ethylcarboimiide hydrochloride (EDC) (25 mM) and pridine (7%, w/w)