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The eluate or cleaved fractions for (GST-GATE16) 2 or mGATE16, respectively, were pooled, concentrated to 2 ml, and purified over a S75 16/600 size-exclusion column in buffer SB (20 mM Tris, 150 mM NaCl, pH 7.5)

Transmission electron microscopy and BODIPY staining Samples collected from each infection stage were fixed in 2.5% ( v/v ) glutaraldehyde at 4 C overnight, and were washed with 0.1 M phosphate buffer saline (PBS), post-fixed in 1% ( w/v ) osmic acid for 2 h, washed again with 0.1 M PBS, dehydrated sequentially with ethanol gradient, and infiltrated with epoxy

QTL analysis of genetic tolerance to iron toxicity in rice ( Oryza sativa L.) by quantification of bronzing score