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Additional biochemical assays were used to test the ability of compounds to inhibit nicotinamide phosphoribosyl transferase (NAMPT) and NAD + -dependent protein deacetylase sirtuin 1 (SIRT1), two enzymes in the NAD + biosynthesis/salvage pathway

Considering that FXR signaling is activated by CA but not by UDCA, the anti-diabetic action of BAs in Insr P1195L/ +/HFD mice might be mediated also by a FXR-independent mechanism