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PBS (177 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 5.5 was used as dilution and running buffer, while PBS (195 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 7.4 was used as regeneration buffer

Co-trimoxazole is a US Food and Drug Administration-approved drug consisting of two antifolates, trimethoprim and sulfamethoxazole, which are on the list of the World Health Organization essential medicines that have been used as effective antimicrobial agents for over 40 years [34, 70]