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Input material was normalized to 20 ng, ribosomal RNA depleted using NEBNext rRNA Depletion Kit (NEB, Human/Mouse/Rat) and strand-specific library preparation used NEBNext Ultra II mRNA kit (NEB) following manufacturers instructions

Symptoms of NF1 caf-au-lait spots, which are tan spots in different sizes and shapes, often found in multiple places freckles, especially those in clusters under the arms or in the groin neurofibromas, or non-cancerous tumors that grow around nerves plexiform neurofibromas, or larger growths that affect the bones, muscles, and other tissue Lisch nodules, or growths that affect the colored part of the eyes (iris) a type of tumor in the optic nerve known as a glioma bone problems, including a curved spine (scoliosis) or leg bones learning issues in childhood, including attention deficit hyperactivity disorder (ADHD) or challenges with reading or math size differences, including a larger head or shorter height than average The cause of NF1 is a genetic mutation