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doi: 10.3390/nu14142869 47 FerrariSMFallahiPDi BariFVitaRBenvengaSAntonelliA

Gene expression analysis The measurement of gene expression in the axes of apple embryos was done using Bio-Rad CFX Connect Real-Time PCR Detection System Total RNA was isolated using RNAzol RT (Sigma, R4533), according to the manufacturers guidelines

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