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Bioassay-guided isolation of the chloroform fraction yielded (E)-4-(3,4-dimethoxyphenyl) but-3-en-1-ol as the active compound (Ramadhan et al., 2020)

In other words, Jupiter is flatter at its poles than at its equator, the result of its fast rotation (just under 10 hours) creating an equatorial bulge

So I went to my local sports PT which had treated me in the past and since my regular physio was on vacation I had a session with another PT

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)