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Isolated hepatocytes were seeded in plates and cultured in DMEM containing 10% fetal bovine serum at 37 C with 5% CO 2

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These shifts can disrupt nutrient absorption and metabolism, interfere with insulin signaling, and enhance inflammatory responses, thus heightening T2DM risk ( Overview of AMPK signaling pathways Adenylate-activated protein kinase (AMPK), also known as AMP-dependent protein kinase, is a pivotal element in diabetes research and other metabolism-related disorders

Ethics statement All aspects of the animal studies and all protocols met the accepted criteria for the care and experimental use of laboratory animals, and were approved by the Animal Research Committee of the 1st Faculty of Medicine, Charles University in Prague (under registration No