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To generate the mixture henceforth described as GAP, appropriate volumes of three amino acids (glutamate source (i.e NaPCA) , cystine, and glycine) were combined in Hank's Balanced Salt Solution (Sigma) such that the glutamate source, cystine, and glycine were in a 0.5: 1: 1 weight ratio

These findings were confirmed by the analysis of the Delocalization Indices (D.I., Fig.2), which measure the average number of electron pairs delocalized (shared) between two atoms

67), S47S53 (2011)

The propionyl CoA is converted in three enzymatic steps, one of which is one of only two reactions in Humans that required vitamin B 12 , to Succinyl CoA, an intermediate of the TCA cycle