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To verify that our individual-centric spheroids, that contain HepG2-derived hepatocyte-like cells, hepatic stellate cells and monocyte-derived macrophages and dendritic cells, do not overgrowth, we generated spheroids using blood sera from 2 females and 2 males and then monitored cell proliferation by measuring Ki67 up to 16 days post-seeding (Figure 3A)

The plasmid pSV2-neo contains a dominant selectable marker, the neo gene from Tn5 encoding an enzyme that confers resistance to a group of antibiotics including G418

Therefore, this study potentially needs a richer population composition and sample size to verify and guarantee its effectiveness and usability

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