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The distinction matters enormously: it is the difference between exposing cells to a free synthetic peptide of uncertain stability and having living tissue produce the E-domain in its natural molecular and cellular context

Measurements: Safety assessments included a physical examination, vital signs, and clinical laboratory parameters including hematology, comprehensive metabolic panel, and prothrombin time-partial thromboplastin time were obtained at baseline and at the end of the study

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In the first step, glycerol is phosphorylated to glycerol 3-phosphate by the enzyme glycerol kinase (EC 2.7.1.30), with the consumption of one ATP