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Fifty-four male ICR mice (1822 g) were purchased from the Experimental Animal Center of Yangzhou University (Yangzhou, China) and housed under controlled environmental conditions (2327 C and 12 h light/dark cycle) with free access to standard diet and water

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Since the surface of an IgG antibody contains up to 80100 lysine residues, of which approximately 3040 are solvent-accessible, NHS ester chemistry can be used to load cytotoxic payloads

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