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23 It is well established that glutathione can be transported across the intestinal epithelium after oral supplementation, 9,19 yet the fate of orally administered GSH is to be resolved as it is readily oxidized within the human body

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To determine the total glutathione (GSH + 2GSSG) concentration, the following stock solutions were freshly prepared in KPE (0.1 M potassium phosphate, 5 mM EDTA, pH 7.5): 5,5-dithio- bis -2-nitrobenzoic acid (DNTB) at 0.6 mg/mL, NADPH at 0.6 mg/mL and glutathione reductase at 3 units/mL