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Molecular and clinical heterogeneity in pyruvate kinase deficiency in India

It was stated, but unsubstantiated by data, that dietary analysis showed that dimethipin was stable in the diet for 7 days and that the mixture of dimethipin and basal diet was satisfactorily homogeneous

To avoid metHb accumulation, reducing pathways mediated by cytochrome b5 or flavin coupled with NADH- or NADPH-dependent metHb reductases (MHbR), respectively, keep the level of metHb in erythrocytes at less than 1% of the total Hb under normal conditions (48)

After the reduction, a second alkylation step was carried out by adding 5 l of 100 mM d5-NEM