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The crude product was purified by silica gel column chromatography with the eluent being a 3:2 solvent mixture of ethyl acetate/hexane to afford (S)-4-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-5-(tert-butoxy)-5-oxopentanoic acid (3.10 g, 99%) as a white solid.1H NMR (300 MHz, CDCl3) : Carboxylic acid peak (1H) not observed (due to broad signal), 7.76 (d, J = 7.5 Hz, 2H), 7.61-7.58 (m, 2H), 7.42-7.26 (m, 4H), 5.45 (d, J = 7.8 Hz, 1H), 4.48-4.19 (m, 4H), 2.51-2.34 (m, 2H), 2.28-2.14 (m, 1H), 1.99 -1.88 (m, 1H), 1.47 (s, 9H)