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6,7 Paraffin sections of tumours were incubated with respective primary antibodies against human -SMA (-smooth muscle actin) (1:800), cytokeratin (1:75), PCNA (proliferating cell nuclear antigen 1:200), Ki-67 (1:333), DCLK1 (1:500), E-cadherin (1:200) and vimentin (1:3000), followed by incubation with relevant secondary antibodies and DAB visualisation

A mechanistic study revealed that Pue-TSA combination therapy markedly activated the Nrf2/ARE signaling pathway, increased the nuclear expression of Nrf2, decreased its cytoplasmic expression and markedly upregulated HO-1 and NAD(P)H oxidoreductase 1, while downregulating Keap1 (143)