glutathione-s-transferase tag purification GST-tagged Protein Kit Purification of proteins fused to

Despite growing public awareness of alcohol-induced liver toxicity, alcohol consumption continues to rise [1, 13]

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Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Du et al., 2022) on a QIAGEN Q-Rex machine (Qiagen, Germany)