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This could be achieved by using most prevailing method by physically shielding the pDNA through the electrostatic binding of the cationic polymer PEI

Ochoa et al., 2018)

Samples were run on NuPAGE 412% Bis-Tris gels using MOPS running buffer (Invitrogen)

From another point, the precise role of autoantibodies against -cell autoantigens, e.g., insulin-specific autoantibodies, insulinoma antigen, zinc transporter-8, and glutamic acid decarboxylase, in the pathogenesis of T1D remains unclear