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After incubation for 1 h, the DCFH-DA probe (10 M) was added and incubated in the dark for 30 min to detect ROS

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Then it was ground in liquid nitrogen, 1 ml of TRIzol (NR0002, Regen, China) was added to the ground sample for lysis, chloroform was added to dissociate the nucleoprotein complex completely, the upper aqueous layer was transferred to isopropanol after centrifugation, and the upper aqueous layer was added to 70% ethanol after centrifugation and washed twice

This finding was consistent with the TEM findings observing regeneration on the 28 th day